Regulatory

Part:BBa_K1051301:Design

Designed by: Kequan Lin   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-30)


clb2 promoter (during G2 phase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 463
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 437
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We get the sequence of CLN2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast genome using PCR .The primers are as follows : 

upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG

downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTACTATAAGATCAATGAAGAGA


Source

CTAGC ATCTC ATAGC ATGGG CATAGAGTTTCAGAAATTTTGCTCTTAATGGAAAATATAA CCTCTTTGGGGAAAAGAGAAATAGATCCTTTCTCAAAAATTTAGAAAGGAAAAAAAATCA AAATATAATAAAATAGATTTTTTTTAGGTTAATTTTTTGCCAATTTAGCTAGACTTTTGA AAAGATCCAAAAATTTACTAAAATTGAATCTTTCTGGTATTAATTTTGTCCTATACCTCA AGAAGTAAAGTATTCTCGAATTGATACTTCTGAAAAGCGAAAGAAATATAGCTAAAGGAT ACTGGTTATTTATTCTTTCTTTTTTTCTTTCTTTTTGATTGAGCCAGTGTATAATAAAAA CCTACTGCGAGCTATTCAATAAATTGACATAGAATATCTGTTGAAGCGGTTCTTTGATTG AGCATCTGTTTTCCTTTTGTATTTTTGTCCTTTTAGATTCTTTTAACGACATTGGAGAAA TTTTAATCATTGTATAAAAACTTCATTCGCTCGTTTGTCAGAAGTTTTTTTATAACTTGC TATTTCCTTCCAAGAAGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAG

References